---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
layout: post
title: Samples Received - Marinelli Shellfish Company C.gigas and C.sikamea Oysters
date: '2019-10-30 14:33'
tags:
  - Crassostrea gigas
  - pacific oyster
  - Crassostrea sikamea
  - Kumamoto
categories:
  - 2019
  - Miscellaneous
---
Steven was recently contacted by Marinelli Shellfish Company to see if we could help them determine if some oysters they had were _Crassostrea gigas_ (Pacific oyster) or _Crassostrea sikamea_ (Kumamoto). Steven knows of a paper with primer sequences to use with qPCR for this specific determination.

They sent ~12 of:

- known _Crassostrea gigas_

- known _Crassostrea sikamea_

- unknown

We collected mantle tissue from 12 of each group. Samples were labeled in the following fashion:

- _C.gigas_ 1191-SS ## (known _Crassostrea gigas_)

- _C.sikamea_ CA5SS ## (known _Crassostrea sikamea_)

- _C.sikamea_ 1191-SS ## (unknown)


 Samples were stored at -80<sup>o</sup>C in Rack 2, Column 4, Row 5:

- [-80<sup>o</sup>C Inventory](http://b.link/srlab-80C)

NOTE: Only 11 samples were collected for _C.sikamea_ CA5SS.


---

#### Paperwork

![paperwork included with shipment](https://github.com/RobertsLab/sams-notebook/blob/master/images/20191030_marinelli_shellfish-01.jpg?raw=true)

---

#### _C.gigas_ 1191-SS ## (known _Crassostrea gigas_)

![bag and label of C.gigas 1191-SS ## (known _Crassostrea gigas_)](https://github.com/RobertsLab/sams-notebook/blob/master/images/20191030_marinelli_shellfish-02.jpg?raw=true)

![C.gigas 1191-SS ## (known _Crassostrea gigas_ out of bag](https://github.com/RobertsLab/sams-notebook/blob/master/images/20191030_marinelli_shellfish-06.jpg?raw=true)

---

#### _C.sikamea_ CA5SS ## (known _Crassostrea sikamea_)

![bag and label of C.sikamea CA5SS ## (known _Crassostrea sikamea_)](https://github.com/RobertsLab/sams-notebook/blob/master/images/20191030_marinelli_shellfish-03.jpg?raw=true)

![_C.sikamea_ CA5SS ## (known _Crassostrea sikamea_ out of bag](https://github.com/RobertsLab/sams-notebook/blob/master/images/20191030_marinelli_shellfish-07.jpg?raw=true)

---

#### _C.sikamea_ 1191-SS ## (unknown)


![bag and label of C.sikamea 1191-SS ## (unknown)](https://github.com/RobertsLab/sams-notebook/blob/master/images/20191030_marinelli_shellfish-04.jpg?raw=true)



![C.sikamea 1191-SS ## (unknown) out of bag](https://github.com/RobertsLab/sams-notebook/blob/master/images/20191030_marinelli_shellfish-05.jpg?raw=true)