--- author: Sam White toc-title: Contents toc-depth: 5 toc-location: left layout: post title: Data Received - C.bairdi RNAseq Day9-12-26 Infected-Uninfected date: '2019-10-24 09:56' tags: - tanner crab - RNAseq - Chionoecetes bairdi categories: - 2019 - Tanner Crab RNAseq - Data Received --- Previously, we "received" this data, but it turns out it was incomplete ([see 20191003](https://robertslab.github.io/sams-notebook/posts/2019/2019-10-03-Data-Received---C.bairdi-RNAseq-Day9-12-26-Infected-Uninfected/)). Today, we _finally_ received _all_ the RNAseq data (>50M reads per samples) back from NWGC that we submitted on [20190521](https://robertslab.github.io/sams-notebook/posts/2019/2019-05-21-Sample-Submission---Tanner-Crab-Infected-vs-Uninfected-RNAseq/)! The second round of data is _in addition_ to the data we received on [20191003](https://robertslab.github.io/sams-notebook/posts/2019/2019-10-03-Data-Received---C.bairdi-RNAseq-Day9-12-26-Infected-Uninfected/). So, to simplify some of the data management and downstream processing of these files, I decided to concatenate the two sets of file. Concatenation is documented in this Jupyter Notebook (GitHub): - [20191024_swoose_cbai_fastq_concatenation.ipynb](https://github.com/RobertsLab/code/blob/master/notebooks/sam/20191024_swoose_cbai_fastq_concatenation.ipynb) Here's a table with the library names and the FastQ naming schemes. | NWGC Sample ID | Investigator Sample ID | |----------------|------------------------| | ~~329772~~ | ~~D9_infected~~ | | ~~329773~~ | ~~D9_uninfected~~ | | 329774 | D12_infected | | 329775 | D12_uninfected | | 329776 | D26_infected | | 329777 | D26_uninfected | The two samples with strikeouts above failed sequencing. See the previous post from [20191003](https://robertslab.github.io/sams-notebook/posts/2019/2019-10-03-Data-Received---C.bairdi-RNAseq-Day9-12-26-Infected-Uninfected/) about data delivery for all the info on those two samples. --- Here's the list of FastQ files: - 329774_S1_L001_R1_001.fastq.gz - 329774_S1_L001_R2_001.fastq.gz - 329774_S1_L002_R1_001.fastq.gz - 329774_S1_L002_R2_001.fastq.gz - 329775_S2_L001_R1_001.fastq.gz - 329775_S2_L001_R2_001.fastq.gz - 329775_S2_L002_R1_001.fastq.gz - 329775_S2_L002_R2_001.fastq.gz - 329776_S3_L001_R1_001.fastq.gz - 329776_S3_L001_R2_001.fastq.gz - 329776_S3_L002_R1_001.fastq.gz - 329776_S3_L002_R2_001.fastq.gz - 329777_S4_L001_R1_001.fastq.gz - 329777_S4_L001_R2_001.fastq.gz - 329777_S4_L002_R1_001.fastq.gz - 329777_S4_L002_R2_001.fastq.gz All files have been added to nightingales/C_bairdi: - [nightingales/C_bairdi](https://owl.fish.washington.edu/nightingales/C_bairdi/) Will update nightingales/C_bairdi/readme.txt and [Nightingales Google sheet](http://b.link/nightingales)