---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
layout: post
title: MBD Enrichment - DNA Quantification of C.virginica MBD Samples from 20190312
date: '2019-03-19 10:28'
tags:
  - MBD
  - BSseq
  - DNA quantification
  - Qubit 3.0
  - 1x high sensitivity dsDNA
  - Crassostrea virginica
  - eastern oyster
categories:
  - 2019
  - Miscellaneous
---
[Finished MBD enrichment on 20190312](https://robertslab.github.io/sams-notebook/posts/2019/2019-03-12-MBD-Enrichment---Ethanol-Precipitation-of-C.virginica-MBD-samples/) using the MethylMiner kit. Since we were out of Qubit assay tubes, I could not quantify these samples when I initially completed the ethanol precipitation. Tubes are in, so I went forward with quantification using the Roberts' Lab Qubit 3.0 and the 1x High Sensitivity dsDNA assay.

Used 1uL of each sample.

---

# RESULTS

Qubit data (Google Sheet):

- [20190319_qubit_DNA_Cvir_MBD](https://docs.google.com/spreadsheets/d/1qhd_wyxJn234Lw8Wk9F6dUfjIWdVHfck_ON7tAi2FXo/edit?usp=sharing)

Concentrations range from 0.702ng/uL to 3.44ng/uL. This corresponds to a recovery rate range of 0.7% - 3.44% (17.6ng - 86ng). Although that low sample is pretty low, I'm fairly certain ZymoResearch can handle sample input this low without issue. Will get a PO number generated and get these sent off to Zymo for bisulfite conversion, library prep and sequencing.
