---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2018-10-15 21:02:16+00:00
layout: post
slug: data-received-chionoecetes-bairdi-rnaseq-fastqc-analysis
title: Data Received - Chionoecetes bairdi RNAseq & FastQC Analysis
categories:
  - 2018
  - Miscellaneous
tags:
  - Chionoecetes bairdi
  - FASTQC
  - hemolymph
  - md5
  - multiqc
  - NovaSeq
  - RNAseq
  - tanner crab
---

We received [Grace's 100bp PE NovaSeq (Illumian) RNAseq data](https://grace-ac.github.io/Pooled-sample-handed-toNWGC/) from the Northwest Genomics Center today.

Data was downloaded via their Aspera browser plugin and rsynced to:





  * [Owl/nightingales/C_bairdi](https://owl.fish.washington.edu/nightingales/C_bairdi/)



MD5 checksums were generated (md5sum on Ubuntu):



  * [checksums.md5](https://owl.fish.washington.edu/nightingales/C_bairdi/checksums.md5)




    
    <code>
    321ec408ba7e0f0be1929ca44871f963  304428_S1_L001_R1_001.fastq.gz
    b95c69f755c9c42d9203429119d4234d  304428_S1_L001_R2_001.fastq.gz
    a0fd8db312057dedd480231d4d125fd3  304428_S1_L002_R1_001.fastq.gz
    c6e70ef7f3c8a866851a1b9453aef36a  304428_S1_L002_R2_001.fastq.gz
    </code>



FastQC analysis was run, followed by MultiQC.

Output folder ([gannet/Atumefaciens](https://gannet.fish.washington.edu/Atumefaciens)):





  * [20181015_Cbairdi_fastqc/](https://gannet.fish.washington.edu/Atumefaciens/20181015_Cbairdi_fastqc/)



MultiQC Report (HTML):



  * [20181015_Cbairdi_fastqc/multiqc_report.html](https://gannet.fish.washington.edu/Atumefaciens/20181015_Cbairdi_fastqc/multiqc_report.html)



Nightingales spreadsheet was updated with file info and FastQC info:



  * [Nightingales (Google Sheet)(https://docs.google.com/spreadsheets/d/1_XqIOPVHSBVGscnjzDSWUeRL7HUHXfaHxVzec-I-8Xk/edit?usp=sharing)