---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2018-08-01 21:23:02+00:00
layout: post
slug: bioanalyzer-tanner-crab-rna-isolated-with-rneasy-plus-mini-kit
title: Bioanalyzer - Tanner Crab RNA Isolated with RNeasy Plus Mini Kit
categories:
  - 2018
  - Miscellaneous
tags:
  - bioanalyzer
  - Chionoecetes bairdi
  - electropherogram
  - RNA
  - RNA Pico 6000
  - tanner crab
---

Ran the four Tanner crab RNA samples that [I isolated yesterday](https://robertslab.github.io/sams-notebook/posts/2018/2018-07-31-rna-isolation-tanner-crab-hemolymph-using-rneasy-plus-mini-kit/) on the Seeb Lab Bioanalyzer 2100 (Agilent) using the RNA Pico 6000 Kit.

Samples were run following kit protocol:





  * Chip priming station in Position C with syringe clip at top position



  * RNA denatured at 70C for 2mins and stored on ice.



  * RNA ladder aliquot was from 20160826 by Hollie Putnam.






* * *





# RESULTS





Bioanalyzer data file (XAD):





  * [Eukaryote Total RNA Pico_2018-08-01_11-45-07.xad](https://owl.fish.washington.edu/Athaliana/20180801_bioanalyzer_crab_RNA/Eukaryote%20Total%20RNA%20Pico_2018-08-01_11-45-07.xad)



ELECTROPHEROGRAMS:

![](https://owl.fish.washington.edu/Athaliana/20180801_bioanalyzer_crab_RNA/20180801_bioanalyzer_crab_electropherogram.jpg)



* * *



GEL REPRESENATATIONS
![](https://owl.fish.washington.edu/Athaliana/20180801_bioanalyzer_crab_RNA/20180801_bioanalyzer_crab_gel.jpg)



* * *



These results look great to me. Clear, defined peaks/bands, representing ribosomal RNA.

Oddly, one sample (crab_506) appears to be shifted, relative to the other three, despite exhibiting the same peak/banding pattern. Not sure what would cause something like this; contaminants?

Regardless, we finally have clean RNA and have a usable Bioanalyzer profile to use for reference for crab RNA.

NOTE: The lanes marked with red on the gel representation image indicate that a ribosomal integrity number (RIN) could not be calculated. This is to be expected! The RIN is based on the expectation of two rRNA bands. The anomaly is sample crab_451 - a RIN was actually determined for that sample!

Will likely move forward with additional RNA isolations using the RNeasy Plus Kit (Qiagen).
