--- author: Sam White toc-title: Contents toc-depth: 5 toc-location: left date: 2018-03-29 17:57:42+00:00 layout: post slug: data-recived-crassostrea-virginica-mbd-bs-seq-from-zymoresearch title: Data Received - Crassostrea virginica MBD BS-seq from ZymoResearch categories: - 2018 - Miscellaneous tags: - BS-seq - Crassostrea virginica - Eastern oyster - Illumina - MBD - ZymoResearch --- Received the sequencing data from ZymoResearch for the Crassostrea virginica gonad MBD DNA that was [sent to them on 20180207](https://robertslab.github.io/sams-notebook/posts/2018/2018-02-07-ethanol-precipitation-dna-quantification-c-virginica-mbd-dna-from-yaamini/) for bisulfite conversion, library construction, and sequencing. Gzipped FASTQ files were: 1. downloaded to [Owl/nightingales/C_virginica](https://owl.fish.washington.edu/nightingales/C_virginica) 2. MD5 checksums verified 3. MD5 checksums appended to the [checksums.md5 file](https://owl.fish.washington.edu/nightingales/C_virginica/checksums.md5) 4. [readme.md file updated](https://owl.fish.washington.edu/nightingales/C_virginica/readme.md) 5. Updated [nightingales Google Sheet](https://docs.google.com/spreadsheets/d/1_XqIOPVHSBVGscnjzDSWUeRL7HUHXfaHxVzec-I-8Xk/edit?usp=sharing) Here's the list of files received: [code lang=text] zr2096_10_s1_R1.fastq.gz zr2096_10_s1_R2.fastq.gz zr2096_1_s1_R1.fastq.gz zr2096_1_s1_R2.fastq.gz zr2096_2_s1_R1.fastq.gz zr2096_2_s1_R2.fastq.gz zr2096_3_s1_R1.fastq.gz zr2096_3_s1_R2.fastq.gz zr2096_4_s1_R1.fastq.gz zr2096_4_s1_R2.fastq.gz zr2096_5_s1_R1.fastq.gz zr2096_5_s1_R2.fastq.gz zr2096_6_s1_R1.fastq.gz zr2096_6_s1_R2.fastq.gz zr2096_7_s1_R1.fastq.gz zr2096_7_s1_R2.fastq.gz zr2096_8_s1_R1.fastq.gz zr2096_8_s1_R2.fastq.gz zr2096_9_s1_R1.fastq.gz zr2096_9_s1_R2.fastq.gz [/code] Here's the sample processing history: * [Tissue received from K. Lotterhos](https://robertslab.github.io/sams-notebook/posts/2017/2017-10-03-samples-received-c-virginica-gonad-tissue-from-katie-lotterhos/) * [DNA isolation](https://robertslab.github.io/sams-notebook/posts/2017/2017-11-14-dna-isolation-quantification-c-virginica-gonad-gdna/) * [Yaamini MBD Day 1](https://yaaminiv.github.io/Virginica-MBDSeq/) * [Yaamini MBD Day 2](https://yaaminiv.github.io/Virginica-MBDSeq-Day2/) * [Yaamini MBD Day 3](https://yaaminiv.github.io/Virginica-MBDSeq-Day3/) * [Yaamini MBD Day 4](https://yaaminiv.github.io/Virginica-MBDSeq-Day4/) * [EtOH precip, quantification, shipped](https://robertslab.github.io/sams-notebook/posts/2018/2018-02-07-ethanol-precipitation-dna-quantification-c-virginica-mbd-dna-from-yaamini/)