---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2018-03-29 17:57:42+00:00
layout: post
slug: data-recived-crassostrea-virginica-mbd-bs-seq-from-zymoresearch
title: Data Received - Crassostrea virginica MBD BS-seq from ZymoResearch
categories:
  - 2018
  - Miscellaneous
tags:
  - BS-seq
  - Crassostrea virginica
  - Eastern oyster
  - Illumina
  - MBD
  - ZymoResearch
---

Received the sequencing data from ZymoResearch for the <em>Crassostrea virginica</em> gonad MBD DNA that was [sent to them on 20180207](https://robertslab.github.io/sams-notebook/posts/2018/2018-02-07-ethanol-precipitation-dna-quantification-c-virginica-mbd-dna-from-yaamini/) for bisulfite conversion, library construction, and sequencing.

Gzipped FASTQ files were:





  1. downloaded to [Owl/nightingales/C_virginica](https://owl.fish.washington.edu/nightingales/C_virginica)


  2. MD5 checksums verified


  3. MD5 checksums appended to the [checksums.md5 file](https://owl.fish.washington.edu/nightingales/C_virginica/checksums.md5)


  4. [readme.md file updated](https://owl.fish.washington.edu/nightingales/C_virginica/readme.md)


  5. Updated [nightingales Google Sheet](https://docs.google.com/spreadsheets/d/1_XqIOPVHSBVGscnjzDSWUeRL7HUHXfaHxVzec-I-8Xk/edit?usp=sharing)



Here's the list of files received:

[code lang=text]
zr2096_10_s1_R1.fastq.gz
zr2096_10_s1_R2.fastq.gz
zr2096_1_s1_R1.fastq.gz
zr2096_1_s1_R2.fastq.gz
zr2096_2_s1_R1.fastq.gz
zr2096_2_s1_R2.fastq.gz
zr2096_3_s1_R1.fastq.gz
zr2096_3_s1_R2.fastq.gz
zr2096_4_s1_R1.fastq.gz
zr2096_4_s1_R2.fastq.gz
zr2096_5_s1_R1.fastq.gz
zr2096_5_s1_R2.fastq.gz
zr2096_6_s1_R1.fastq.gz
zr2096_6_s1_R2.fastq.gz
zr2096_7_s1_R1.fastq.gz
zr2096_7_s1_R2.fastq.gz
zr2096_8_s1_R1.fastq.gz
zr2096_8_s1_R2.fastq.gz
zr2096_9_s1_R1.fastq.gz
zr2096_9_s1_R2.fastq.gz
[/code]

Here's the sample processing history:



  * [Tissue received from K. Lotterhos](https://robertslab.github.io/sams-notebook/posts/2017/2017-10-03-samples-received-c-virginica-gonad-tissue-from-katie-lotterhos/)


  * [DNA isolation](https://robertslab.github.io/sams-notebook/posts/2017/2017-11-14-dna-isolation-quantification-c-virginica-gonad-gdna/)


  * [Yaamini MBD Day 1](https://yaaminiv.github.io/Virginica-MBDSeq/)


  * [Yaamini MBD Day 2](https://yaaminiv.github.io/Virginica-MBDSeq-Day2/)


  * [Yaamini MBD Day 3](https://yaaminiv.github.io/Virginica-MBDSeq-Day3/)


  * [Yaamini MBD Day 4](https://yaaminiv.github.io/Virginica-MBDSeq-Day4/)


  * [EtOH precip, quantification, shipped](https://robertslab.github.io/sams-notebook/posts/2018/2018-02-07-ethanol-precipitation-dna-quantification-c-virginica-mbd-dna-from-yaamini/)


