---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2018-02-07 20:36:23+00:00
layout: post
slug: ethanol-precipitation-dna-quantification-c-virginica-mbd-dna-from-yaamini
title: Ethanol Precipitation & DNA Quantification - C. virginica MBD DNA from Yaamini
categories:
  - 2018
  - Samples Submitted
tags:
  - Crassostrea virginica
  - DNA Quantification
  - Eastern oyster
  - EtOH precipitation
  - MethylMiner Methylated DNA Enrichment Kit
  - Qubit 3.0
  - Qubit dsDNA HS
  - ZymoResearch
---

Finished the ethanol precipitation as described in the [MethylMiner (Invitrogen) manual](https://github.com/RobertsLab/resources/blob/master/protocols/Commercial_Protocols/Invitrogen_MethylMiner_Manual.pdf) which Yaamini had previously initiated: [https://yaaminiv.github.io/Virginica-MBDSeq-Day4/](https://yaaminiv.github.io/Virginica-MBDSeq-Day4/)

Samples were resuspended in 25μL of Buffer EB (Qiagen) and transferred to 0.5mL snap cap tubes. All tubes were labeled as: MBD CV #

Quantified the _Crassostrea virginica_ MBD-enriched DNA with the Qubit 3.0 (ThermoFisher) and the Qubit dsDNA High Sensitivity (HS) Kit (ThermoFisher).

Used 1uL of template DNA.

Results:

Quantification Spreadsheet (Google Sheet):[20180207_qubit_DNA_HS_MBD_virginica](https://docs.google.com/spreadsheets/d/1p13VsqTMrynvWIfokPVAwqaL78_GA6QtTZ0NAgLVfSI/edit?usp=sharing)

One sample (MBD CV 106) may not be usable due to low yield. However, the remainder should work fine.

I've sent them all to ZymoResearch for bisulfite treatment, library construction, and Illumina sequencing.

FedEx tracking: 771429590026
