---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2018-01-11 21:48:21+00:00
layout: post
slug: restriction-digestion-mspi-on-crassotrea-virginica-gdna
title: Restriction Digestion - MspI on Crassotrea virginica gDNA
categories:
  - 2018
  - Miscellaneous
tags:
  - Crassostrea virginica
  - Eastern oyster
  - MspI
  - restriction digestion
---

Digested two 1.5μg aliquots of _Crassostrea virginica_ [isolated 20171211](https://robertslab.github.io/sams-notebook/posts/2017/2017-12-11-dna-isolation-quantification-crassotrea-virginica-mantle-gdna/), as part of the project we're doing with Qiagen.

Digestion reactions:

<table >

<tr >
  Component
  Volume(μL)
</tr>

<tbody >
<tr >
  
<td >DNA (1.5μg)
</td>
  
<td >25.7
</td>
</tr>
<tr >
  
<td >10x CutSmart Buffer (NEB)
</td>
  
<td >5.0
</td>
</tr>
<tr >
  
<td >Water
</td>
  
<td >17.3
</td>
</tr>
<tr >
  
<td >MspI (NEB)
</td>
  
<td >2
</td>
</tr>
<tr >
  
<td >**TOTAL**
</td>
  
<td >**50**
</td>
</tr>
</tbody>
</table>

MspI info:





  * NEB R0106T (100,000U/mL; rec'd 20171214)



Reactions were carried out in 0.5mL snap-cap PCR tubes and incubated for 15mins @ 37oC in a PTC-200 thermalcycler (MJ Research), no heated lid.

Samples will be subjected to a phenol:chloroform extraction for cleanup.
