---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2018-01-11 23:41:35+00:00
layout: post
slug: dna-quantification-mspi-digested-crassostrea-virginica-gdna
title: DNA Quantification - MspI-digested Crassostrea virginica gDNA
categories:
  - 2018
  - Miscellaneous
tags:
  - Crassostrea virginica
  - Eastern oyster
  - Qubit 3.0
  - Qubit dsDNA BR
---

Quantified the two MspI-digested DNA samples for the Qiagen project [from earlier today](https://robertslab.github.io/sams-notebook/posts/2018/2018-01-11-phenolchlorofo…-earlier-today/) with the Qubit 3.0 (ThermoFisher).

Used the Qubit dsDNA Broad Range (BR) Kit (ThermoFisher).

Used 1μL of DNA from each sample (including [undigested gDNA from initial isolation 20171211](https://robertslab.github.io/sams-notebook/posts/2017/2017-12-11-dna-isolation-quantification-crassotrea-virginica-mantle-gdna/)

Results:

Quantification (Google Sheet): [20180111_qubit_DNA_MspI_virginica](https://docs.google.com/spreadsheets/d/1SxPvPQLx2sXNSOSeT4bwEkqqSafSPaupKmy48OlJyow/edit?usp=sharing)

Yields are good and are sufficient for submission to Qiagen:

MspI_virginica_01 - 53.4ng/μL (1335ng; 89% recovery after phenol/chloroform/EtOH precip)
MspI_virginca_02 - 31.0ng/μL (775ng; ~52% recovery after phenol/chloroform/EtOH precip)