--- author: Sam White toc-title: Contents toc-depth: 5 toc-location: left date: 2017-09-18 21:50:13+00:00 layout: post slug: genome-assembly-olympia-oyster-pacbio-minimapminiasmracon-2 title: Genome Assembly - Olympia oyster PacBio minimap/miniasm/racon categories: - 2017 - Olympia Oyster Genome Sequencing tags: - jupyter notebook - miniasm - olympia oyster - Ostrea lurida - PacBio - racon --- In this [GitHub Issue](https://github.com/RobertsLab/project-olympia.oyster-genomic/issues/29), Steven had suggested I try out the minimap/miniasm/racon pipeline for assembling our Olympia oyster PacBio data. I followed the pipeline described by this paper: [https://matzlab.weebly.com/uploads/7/6/2/2/76229469/racon.pdf](http://matzlab.weebly.com/uploads/7/6/2/2/76229469/racon.pdf). Previously, ran the first part of the pipeline: [minimap](https://robertslab.github.io/sams-notebook/posts/2017/2017-09-07-genome-assembly-olympia-oyster-pacbio-minimapminiasmracon/) This notebook entry just contains the miniasm execution. Will follow with [racon](https://robertslab.github.io/sams-notebook/posts/2017/2017-09-18-genome-assembly-olympia-oyster-pacbio-minimapminiasmracon-3/). Jupyter Notebook (GitHub): [20170918_docker_pacbio_oly_miniasm0.2.ipynb](https://github.com/sr320/LabDocs/blob/master/jupyter_nbs/sam/20170918_docker_pacbio_oly_miniasm0.2.ipynb)