--- author: Sam White toc-title: Contents toc-depth: 5 toc-location: left date: 2017-09-07 21:39:38+00:00 layout: post slug: genome-assembly-olympia-oyster-pacbio-minimapminiasmracon title: Genome Assembly - Olympia oyster PacBio minimap/miniasm/racon categories: - 2017 - Olympia Oyster Genome Sequencing tags: - jupyter notebook - minimap - olympia oyster - Ostrea lurida - PacBio --- In this [GitHub Issue](https://github.com/RobertsLab/project-olympia.oyster-genomic/issues/29), Steven had suggested I try out the minimap/miniasm/racon pipeline for assembling our Olympia oyster PacBio data. I followed the pipeline described by this paper: [https://matzlab.weebly.com/uploads/7/6/2/2/76229469/racon.pdf](http://matzlab.weebly.com/uploads/7/6/2/2/76229469/racon.pdf). This notebook entry just contains the initial minimap execution. Followed up with [miniasm](https://robertslab.github.io/sams-notebook/posts/2017/2017-09-18-genome-assembly-olympia-oyster-pacbio-minimapminiasmracon-2//robertslab.github.io/sams-notebook/2017/09/18/genome-assembly-olympia-oyster-pacbio-minimapminiasmracon-3/). Jupyter Notebook (GitHub): [20170907_docker_pacbio_oly_minimap2.ipynb](https://github.com/sr320/LabDocs/blob/master/jupyter_nbs/sam/20170907_docker_pacbio_oly_minimap2.ipynb)