---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2017-07-06 22:47:54+00:00
layout: post
slug: data-management-sra-submission-olympia-oyster-uw-pacbio-data-from-20170323
title: Data Management - SRA Submission Olympia Oyster UW PacBio Data from 20170323
categories:
  - 2017
  - Olympia Oyster Genome Sequencing
tags:
  - FASTQ
  - ftp
  - NCBI
  - olympia oyster
  - Ostrea lurida
  - PacBio
  - PRJNA316624
  - SAMN07326085
  - SRA
  - SRR5809355
---

Submitted the FASTQ files from the [UW PacBio Data from 20170323](https://robertslab.github.io/sams-notebook/posts/2017/2017-03-23-data-management-olympia-oyster-pacbio-data-received/) to the [NCBI sequence read archive (SRA)(https://www.ncbi.nlm.nih.gov/sra).

FTP'd the data to NCBI's servers, following their instructions. Briefly,

Change to the directory where the FASTQ files are (`Owl/web/nightingales/O_lurida`) and then initiate an FTP session:


    
    <code>ftp -i ftp-private.ncbi.nlm.nih.gov</code>



Enter provided username/password, change to my designated uploads directory, create new folder dedicate to this particular upload. Then, upload all the files using the `mput` command:


    
    <code>mput *filtered_subreads*</code>



SRA deets are below (assigned FASTQ files to existing BioProject and created a new BioSample). Will update post with SRA number when processing is complete on the NCBI end.

SRA: SRS2339870
Study: SRR5809355
BioProject: [PRJNA316624](https://www.ncbi.nlm.nih.gov/bioproject/PRJNA316624)
BioSample: [SAMN07326085](https://www.ncbi.nlm.nih.gov/biosample/SAMN07326085/)