---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2017-07-05 21:33:55+00:00
layout: post
slug: sample-annotation-olympia-oyster-histology-blocks-from-laura-spencer
title: Sample Annotation - Olympia oyster histology blocks (from Laura Spencer)
categories:
  - 2017
  - Miscellaneous
tags:
  - gonad
  - histology
  - histology blocks
  - histology slides
  - Laura Spencer
  - NF-10 22
  - NF-10-23
  - NF-10-24
  - NF-10-26
  - NF-10-28
  - NF-10-30
  - olympia oyster
  - Ostrea lurida
  - pics
  - SN-10-16
  - SN-10-17
  - SN-10-20
  - SN-10-25
  - SN-10-26
  - SN-10-31
---

I've been asked to isolate RNA from some paraffin-embedded Olympia oyster gonad tissue.

Despite some excellent documentation by Laura Spencer ([images of tissue layouts in histology cassettes](https://github.com/laurahspencer/LabNotebook/blob/master/_posts/2017-04-20-Prepping-Histology-Samples.md) and [a corresponding cassette mapping key file](https://github.com/laurahspencer/O.lurida_Stress/blob/master/Data/2017-April-HistoKey.pdf)), the histology facility seems to have flipped some things around and/or repositioned/split the contents of each cassette. This makes ID-ing the proper tissues tedious and, at times, difficult.

The list of tissues that needs to be processed is listed in this [GitHub Issue #648](https://github.com/sr320/LabDocs/issues/648). I've also added the list below:

NF-10 22
NF-10-23
NF-10-24
NF-10-26
NF-10-28
NF-10-30
SN-10-16
SN-10-17
SN-10-20
SN-10-25
SN-10-26
SN-10-31

Prior to beginning RNA isolations, I have annotated images of the histology blocks and will be waiting for Laura to confirm that my annotations are correct. I will be posting a link to this notebook entry in the GitHub issue listed above for her to view and wait for her confirmation.

UPDATE 201700707 - Laura has indicated that many of my annotations are incorrect. Katie has gone through and made proper identification: [https://github.com/sr320/LabDocs/issues/648#issuecomment-313792588](https://github.com/sr320/LabDocs/issues/648#issuecomment-313792588)



Additionally, as indicated in the GitHub Issue above, [histology block "Oly 14" does not have a corresponding tissue cassette photo](https://github.com/sr320/LabDocs/issues/648#issuecomment-313213160) (containing sample NF-10 26). Without the original image, I don't think I can make an accurate guess on how the tissues are oriented in the resulting two histo blocks (see below).



BLOCKS 5

![](https://eagle.fish.washington.edu/Arabidopsis/Oly5_histo_block.png)(http://eagle.fish.washington.edu/Arabidopsis/Oly5_histo_block.png)



BLOCK 6

![](https://eagle.fish.washington.edu/Arabidopsis/Oly6_histo_block.png)(http://eagle.fish.washington.edu/Arabidopsis/Oly6_histo_block.png)





BLOCK 9

![](https://eagle.fish.washington.edu/Arabidopsis/Oly9_histo_block.png)(http://eagle.fish.washington.edu/Arabidopsis/Oly9_histo_block.png)





BLOCK 10

![](https://eagle.fish.washington.edu/Arabidopsis/Oly10_histo_block.png)(http://eagle.fish.washington.edu/Arabidopsis/Oly10_histo_block.png)





BLOCKS 14 (unable to annotate at time of posting)

![](https://eagle.fish.washington.edu/Arabidopsis/Oly14_histo_blocks_no_annotation.jpg)(http://eagle.fish.washington.edu/Arabidopsis/Oly14_histo_blocks_no_annotation.jpg)







BLOCK 15

![](https://eagle.fish.washington.edu/Arabidopsis/Oly15_histo_block.png)(http://eagle.fish.washington.edu/Arabidopsis/Oly15_histo_block.png)







BLOCK 21

![](https://eagle.fish.washington.edu/Arabidopsis/Oly21_histo_block.png)(http://eagle.fish.washington.edu/Arabidopsis/Oly21_histo_block.png)









BLOCK 22

![](https://eagle.fish.washington.edu/Arabidopsis/Oly22_histo_block.png)(http://eagle.fish.washington.edu/Arabidopsis/Oly22_histo_block.png)
