--- author: Sam White toc-title: Contents toc-depth: 5 toc-location: left date: 2016-12-21 22:49:24+00:00 layout: post slug: sample-submission-geoduck-tissue-gdna-for-illumina-pilot-sequencing-project title: Sample Submission - Geoduck Tissue & gDNA for Illumina Pilot Sequencing Project categories: - 2016 - Geoduck Genome Sequencing - Samples Submitted tags: - geoduck - Illumina - Panopea generosa --- Sent the following samples to Illumina for possible selection in a new pilot sequencing platform they're working on. The 12 samples will be used for RNAseq for genome annotation - numbers indicate desired sequencing priority. Juvenile and larval samples were from Hollie Putnam (see links below for more info). Other tissue was from a single, adult geoduck, collected by [Brent & Steven on 20150811](https://onsnetwork.org/halfshell/2015/08/11/big-day-big-clam/). 1. Gonad 2. Heart 3. Ctenidia 4. Juvenile OA exposure (super low)  ([EPI_115, EPI_116](https://github.com/hputnam/project_juvenile_geoduck_OA/blob/master/Setup_Notes/Sample_List.csv)) 5. Juvenile ambient exposure (ambient treatment) ([EPI_123, EPI_124](https://github.com/hputnam/project_juvenile_geoduck_OA/blob/master/Setup_Notes/Sample_List.csv)) 6. Larvae day 0 ([EPI_74, EPI_75](https://github.com/hputnam/project_larval_geoduck_OA/edit/master/Larval_Sample_Info.csv)) 7. Larvae day 5 ([EPI_99](https://github.com/hputnam/project_larval_geoduck_OA/blob/master/Larval_Sample_Info.csv)) 8. Crystalline style 9. Byssus gland 10. Mantle 11. Labial palps 12. Juvenile OA exposure - low treatment ([EPI_107, EPI_108](https://github.com/hputnam/project_juvenile_geoduck_OA/blob/master/Setup_Notes/Sample_List.csv)) In addition to the above 12 samples, ~1.5μg of geoduck gDNA ([isolated this morning](dna-isolation-geoduck-gdna-for-potential-illumina-initiated-sequencing-project.html)) was sent.