---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2016-03-23 23:45:23+00:00
layout: post
slug: sra-submission-individual-transcriptomic-profiles-of-c-gigas-before-after-heat-shock
title: SRA Submission - Individual Transcriptomic Profiles of C.gigas Before & After
  Heat Shock
categories:
  - 2016
  - SRA Submissions
tags:
  - 2M
  - 2M-HS
  - 4M
  - 4M-HS
  - 6M
  - 6M-HS
  - Crassostrea gigas
  - NCBI
  - Pacific oyster
  - SRA
  - SRP072251
---

RNA-seq experiment conducted by [Claire in 2013](https://claireeolson.blogspot.com/).

She sampled mantle tissue from three adult oysters, allowed them to recover from the sampling (one week?) and then subjected those same oysters to a 1hr heat shock at 40C and collected mantle tissue from them again.

As this is our first Small Read Archive (SRA) submission in many years, I decided to submit these to the SRA due to the small number of samples (6) from the Illumina sequencing we had done to make sure it was manageable.

[An overview of the basic SRA submission process is here](https://robertslab.github.io/sams-notebook/posts/2016/2016-03-23-data-management-sra-submission-overview/).

The current status can be seen in the screen cap below. Current release date is set for a year from now, but will likely bump it up. Need Steven to review the details of the submission (BioProject, Experiment descriptions, etc.) before I initiate the public release. Will update this post with the SRA number once we receive it.

Here's the list of files uploaded to the SRA:

2M-HS_CCGTCC_L001_R1_001.fastq.gz
2M_AGTCAA_L001_R1_001.fastq.gz
4M-HS_GTCCGC_L001_R1_001.fastq.gz
4M_AGTTCC_L001_R1_001.fastq.gz
6M-HS_GTGAAA_L001_R1_001.fastq.gz
6M_ATGTCA_L001_R1_001.fastq.gz

SRA Accession: SRP072251

![](https://eagle.fish.washington.edu/Arabidopsis/20160323_SRA_submission.jpg)(http://eagle.fish.washington.edu/Arabidopsis/20160323_SRA_submission.jpg)