---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2015-12-09 00:48:41+00:00
layout: post
slug: sample-submission-olympia-oyster-mbd-enriched-dna-to-zymoresearch
title: Sample Submission - Olympia oyster MBD-enriched DNA to ZymoResearch
categories:
  - 2015
  - MBD Enrichment for Sequencing at ZymoResearch
  - Olympia oyster reciprocal transplant
  - Samples Submitted
tags:
  - BS-seq
  - hc1_2B
  - hc1_4B
  - hc2_15B
  - hc2_17
  - hc3_1
  - hc3_10
  - hc3_11
  - hc3_5
  - hc3_7
  - hc3_9
  - MBD
  - MBD enrichment
  - MBD-Seq
  - olympia oyster
  - Ostrea lurida
  - ss2_14B
  - ss2_18B
  - ss2_9B
  - ss3_14B
  - ss3_15B
  - ss3_16B
  - ss3_20
  - ss3_3B
  - ss3_4B
  - ss5_18
---

We opted to go with ZymoResearch for this project because they could do the bisulfite treatment and finish the sequencing by the end of January.

Submitted the following 18 _Ostrea lurida_ MBD-enriched gDNA samples to ZymoResearch for bisulfite treatment and subsequent Illumina sequencing (50bp, single read):

<table cellspacing="0" border="0" >
<tbody >
<tr >

<td align="left" height="17" >hc1_2B
</td>
</tr>
<tr >

<td align="left" height="17" >hc1_4B
</td>
</tr>
<tr >

<td align="left" height="17" >hc2_15B
</td>
</tr>
<tr >

<td align="left" height="17" >hc2_17
</td>
</tr>
<tr >

<td align="left" height="17" >hc3_1
</td>
</tr>
<tr >

<td align="left" height="17" >hc3_10
</td>
</tr>
<tr >

<td align="left" height="17" >hc3_11
</td>
</tr>
<tr >

<td align="left" height="17" >hc3_5
</td>
</tr>
<tr >

<td align="left" height="17" >hc3_7
</td>
</tr>
<tr >

<td align="left" height="17" >hc3_9
</td>
</tr>
<tr >

<td align="left" height="17" >ss2_14B
</td>
</tr>
<tr >

<td align="left" height="17" >ss2_18B
</td>
</tr>
<tr >

<td align="left" height="17" >ss2_9B
</td>
</tr>
<tr >

<td align="left" height="17" >ss3_14B
</td>
</tr>
<tr >

<td align="left" height="17" >ss3_15B
</td>
</tr>
<tr >

<td align="left" height="17" >ss3_16B
</td>
</tr>
<tr >

<td align="left" height="17" >ss3_20
</td>
</tr>
<tr >

<td align="left" height="17" >ss3_3B
</td>
</tr>
<tr >

<td align="left" height="17" >ss3_4B
</td>
</tr>
<tr >

<td align="left" height="17" >ss5_18
</td>
</tr>
</tbody>
</table>

The samples will be bisulfite treated, Illumina libraries constructed, multiplexed, and this multiplexed library will be sequenced three times.