---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2015-11-22 15:29:50+00:00
layout: post
slug: ethanol-precipitation-olympia-oyster-mbd
title: Ethanol Precipitation - Olympia oyster MBD
categories:
  - 2015
  - MBD Enrichment for Sequencing at ZymoResearch
  - Olympia oyster reciprocal transplant
tags:
  - EtOH precipitation
  - MethylMiner Methylated DNA Enrichment Kit
  - olympia oyster
  - Ostrea lurida
---

Precipitated [the MBD enriched DNA from yesterday](https://robertslab.github.io/sams-notebook/posts/2015/2015-11-22-mbd-enrichment-sonicated-olympia-oyster-gdna/) according to the MethylMiner Methylated DNA Enrichment Kit (Invitrogen) protocol.

However, since the protocol has two elution steps that are each saved separately from each other for each sample, I did the following to combine the two elution fractions into a single sample:




    
  * Pelleted one elution fraction from each sample

    
  * Discarded supernatant from pelleted sample

    
  * Transferred second elution fraction to the pellet from the first elution fraction

    
  * Pelleted second elution fraction



The rest of the ethanol precipitation procedure was followed per the manufacturer's protocol.

Final pellets were resuspended in 25μL of Buffer EB (Qiagen) and stored @ 4C.

MBD enriched DNA will be quantified tomorrow.