---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2015-11-05 01:46:51+00:00
layout: post
slug: dna-quantification-quality-assessment-oly-2sn-gdna
title: DNA Quantification & Quality Assessment - Oly 2SN gDNA
categories:
  - 2015
  - Olympia oyster reciprocal transplant
tags:
  - bioanalyzer
  - DNA Quantification
  - Fidalgo 2SN
  - gDNA
  - NanoDrop1000
  - olympia oyster
  - Ostrea lurida
---

Comparison of three different approaches to using the E.Z.N.A. Mollusc Kit:




    
  * Fresh isolations by me

    
  * Fresh isolations by Mrunmayee

    
  * Isolations from tissue frozen in buffer by me



Results:

Bioanalyzer Data File (XAD file): [2100 expert_DNA 12000_DE72902486_2015-11-04_15-06-32.xad](https://eagle.fish.washington.edu/Arabidopsis/Bioanalyzer%20Data/2100%20expert_DNA%2012000_DE72902486_2015-11-04_15-06-32.xad)

![](https://eagle.fish.washington.edu/Arabidopsis/20151104_gDNA_oly_2SN_ODs.JPG)(http://eagle.fish.washington.edu/Arabidopsis/20151104_gDNA_oly_2SN_ODs.JPG)

![](https://eagle.fish.washington.edu/Arabidopsis/20151104_gDNA_oly_2SN_plots.JPG)(http://eagle.fish.washington.edu/Arabidopsis/20151104_gDNA_oly_2SN_plots.JPG)

![](https://eagle.fish.washington.edu/Arabidopsis/Bioanalyzer%20Data/20151104_bioanalyzer_electropherograms.jpg)(http://eagle.fish.washington.edu/Arabidopsis/Bioanalyzer%20Data/20151104_bioanalyzer_electropherograms.jpg)

![](https://eagle.fish.washington.edu/Arabidopsis/Bioanalyzer%20Data/20151104_bioanalyzer_gels.jpg)(http://eagle.fish.washington.edu/Arabidopsis/Bioanalyzer%20Data/20151104_bioanalyzer_gels.jpg)

There's a LOT going on here. Will update this entry tomorrow with more info.