--- author: Sam White toc-title: Contents toc-depth: 5 toc-location: left date: 2015-11-05 01:45:57+00:00 layout: post slug: dna-quantification-quality-assessment-geoduck-oly-gdna title: DNA Quantification & Quality Assessment - Geoduck & Oly gDNA categories: - 2015 - Geoduck Genome Sequencing - Olympia Oyster Genome Sequencing tags: - bioanalyzer - DNA Quantification - gDNA - geoduck - NanoDrop1000 - olympia oyster - Ostrea lurida - Panopea generosa --- Quantified the following samples with the Roberts Lab NanoDrop1000 (ThermoFisher) and assessed DNA integrity on the Seeb Lab Bioanalyzer 2100 (Agilent) using the DNA 12000 chip assay: * [geoduck gDNA for genome sequencing](https://robertslab.github.io/sams-notebook/posts/2015/2015-10-30-dna-isolation-geoduck-olympia-oyster-2/) * [Olympia oyster gDNA for genome sequencing](https://robertslab.github.io/sams-notebook/posts/2015/2015-10-30-dna-isolation-geoduck-olympia-oyster-2/) Results: Bioanalyzer Data File (XAD file): [2100 expert_DNA 12000_DE72902486_2015-11-04_15-06-32.xad](https://eagle.fish.washington.edu/Arabidopsis/Bioanalyzer%20Data/2100%20expert_DNA%2012000_DE72902486_2015-11-04_15-06-32.xad) ![](https://eagle.fish.washington.edu/Arabidopsis/20151104_gDNA_geo_oly_ODs.JPG)(http://eagle.fish.washington.edu/Arabidopsis/20151104_gDNA_geo_oly_ODs.JPG) ![](https://eagle.fish.washington.edu/Arabidopsis/20151104_gDNA_geo_oly_plots.JPG)(http://eagle.fish.washington.edu/Arabidopsis/20151104_gDNA_geo_oly_plots.JPG) ![](https://eagle.fish.washington.edu/Arabidopsis/Bioanalyzer%20Data/20151104_bioanalyzer_geoduck_electropherogram.jpg)(http://eagle.fish.washington.edu/Arabidopsis/Bioanalyzer%20Data/20151104_bioanalyzer_geoduck_electropherogram.jpg) ![](https://eagle.fish.washington.edu/Arabidopsis/Bioanalyzer%20Data/20151104_bioanalyzer_oly_electropherogram.jpg)(http://eagle.fish.washington.edu/Arabidopsis/Bioanalyzer%20Data/20151104_bioanalyzer_oly_electropherogram.jpg) ![](https://eagle.fish.washington.edu/Arabidopsis/Bioanalyzer%20Data/20151104_bioanalyzer_geoduck_oly_gels.jpg)(http://eagle.fish.washington.edu/Arabidopsis/Bioanalyzer%20Data/20151104_bioanalyzer_geoduck_oly_gels.jpg) OK, there's a LOT going on here. Will update this notebook with my thoughts sometime tomorrow...