---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2015-11-04 05:50:30+00:00
layout: post
slug: dna-isolations-oly-fidalgo-2sn-ctenidia
title: DNA Isolations – Oly Fidalgo 2SN Ctenidia
categories:
  - 2015
  - Olympia oyster reciprocal transplant
tags:
  - DNA Isolation
  - E.Z.N.A. Mollusc Kit
  - Fidalgo 2SN
  - gDNA
  - olympia oyster
  - Ostrea lurida
---

Isolated DNA from 24 2SN ctenidia samples from [Friday's sampling](https://robertslab.github.io/sams-notebook/posts/2015/2015-10-31-oyster-sampling-oly-fidalgo-2sn-2hl-2nf-reciprocal-transplants-final-samplings/) (#32 - 55). Samples were thawed at RT.

DNA was isolated using the E.Z.N.A. Mollusc Kit (Omega BioTek) according to [the manufacturer’s protocol](https://github.com/sr320/LabDocs/blob/master/protocols/Commercial_Protocols/Omega_Mollusc-DNA-Kit-Combo-May-2013-D3373.pdf) with the following changes:




    
  * Samples were incubated @ 60C for only 1hr, per Steven's recommendation (an attempt to prevent degradation)

    
  * No optional steps were performed

    
  * Used 300μL of MBL Buffer for all samples (this was more than the recovered volume of aqueous phase from each sample)

    
  * Single elution of 50μL



Samples were stored @ -20C in: Oly gDNA Oly Reciprocal Transplant Final Sampling Box #1.

Some notes:


    
  * Total time (including 1hr incubation): 4.5hrs.

    
  * Short incubation time did not completely digest samples

    
  * Partial tissue digestions led to difficulties in recovering entire aqueous phase, post chloroform treatment




