---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2015-10-05 23:32:15+00:00
layout: post
slug: dna-quantification-pooled-geoduck-gdna
title: DNA Quantification - Pooled geoduck gDNA
categories:
  - 2015
  - Geoduck Genome Sequencing
tags:
  - DNA Quantification
  - gDNA
  - geoduck
  - NanoDrop1000
  - Panopea generosa
---

Pooled the gDNA samples [from earlier today](https://robertslab.github.io/sams-notebook/posts/2015/2015-10-05-gdna-isolation-geoduck-adductor-muscle/) & [from 20151002](https://robertslab.github.io/sams-notebook/2015/10/02/dna-isolation-geoduck-olympia-oyster/).

The pooled volume = 260μL

Quantified on the Roberts Lab NanoDrop1000.

Results:

![](https://eagle.fish.washington.edu/Arabidopsis/20151005_gDNA_geoduck_pool_ODs.JPG)(http://eagle.fish.washington.edu/Arabidopsis/20151005_gDNA_geoduck_pool_ODs.JPG)

![](https://eagle.fish.washington.edu/Arabidopsis/20151005_gDNA_geoduck_pool_plots.JPG)(http://eagle.fish.washington.edu/Arabidopsis/20151005_gDNA_geoduck_pool_plots.JPG)



Besides the weird peak at 240nm, everything looks great - perfect 260/280 & 260/230 ratios.

Yield = 75.92μg

This gDNA has been RNase treated, so the concentration should be semi-accurate. Regardless, even if there's a discrepancy of 50%, this should provide enough additional DNA for BGI to work with.

Will run on gel to evaluate integrity and then send to BGI.
