---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2015-09-18 00:10:38+00:00
layout: post
slug: agarose-gel-geoduck-olympia-oyster-gdna-integrity-check-4
title: Agarose Gel - Geoduck & Olympia oyster gDNA Integrity Check
categories:
  - 2015
  - Geoduck Genome Sequencing
  - Olympia Oyster Genome Sequencing
tags:
  - gDNA
  - gel
  - geoduck
  - O'geneRuler DNA Ladder Mix
  - olympia oyster
  - Ostrea lurida
  - Panopea generosa
---

Ran a 0.8% agarose, 1x modified TAE gel (w/EtBr) with [geoduck and Olympia oyster gDNA that was precipitated earlier today](https://robertslab.github.io/sams-notebook/posts/2015/2015-09-17-ethanol-precipitation-geoduck-olympia-oyster-gdna/). Used 5μL of each sample (~500ng).

Results:

[caption id="" align="alignleft" width="255"]![](https://eagle.fish.washington.edu/Arabidopsis/20150917_gel_gDNA_geoduck_oly.jpg)(http://eagle.fish.washington.edu/Arabidopsis/20150917_gel_gDNA_geoduck_oly.jpg) Geoduck gDNA on left. Oly gDNA on right.[/caption]

![](https://raw.githubusercontent.com/sr320/LabDocs/master/protocols/Commercial_Protocols/ThermoFisher_OgeneRuler_DNA_Ladder_Mix_F100439.jpg)(https://raw.githubusercontent.com/sr320/LabDocs/master/protocols/Commercial_Protocols/ThermoFisher_OgeneRuler_DNA_Ladder_Mix_F100439.jpg)



























Overall, the DNA still looks very good. Slight smearing (indicating slight degradation), but the high molecular weight band is very prominent. Will fill out the necessary BGI forms and ship samples out on Monday.


