---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2015-09-16 00:22:52+00:00
layout: post
slug: agarose-gel-geoduck-olympia-oyster-gdna-integrity-check-3
title: Agarose Gel - Geoduck & Olympia Oyster gDNA Integrity Check
categories:
  - 2015
  - Geoduck Genome Sequencing
  - Olympia Oyster Genome Sequencing
tags:
  - gDNA
  - gel
  - geoduck
  - O'geneRuler DNA Ladder Mix
  - olympia oyster
  - Ostrea lurida
  - Panopea generosa
---

Ran 0.8% agarose 1x modified TAE gel stained with EtBr to assess the integrity of [geoduck gDNA](https://robertslab.github.io/sams-notebook/posts/2015/2015-09-15-genomic-dna-isolation-geoduck-adductor-muscle-foot-3/) and [Olympia oyster gDNA](https://robertslab.github.io/sams-notebook/2015/09/15/genomic-dna-isolation-olympia-oyster-adductor-musle-mantle-3/) isolated earlier today.

Ran ~500ng of each sample:

Geoduck adductor muscle 1: 6.8μL

Geoduck adductor muscle 2: 20μL (260ng)

Geoduck foot 1: 16.6μL

Geoduck foot 2: 20μL (200ng)

Oly adductor muscle: 4μL

Oly mantle: 4.7μL

Results:



![](https://raw.githubusercontent.com/sr320/LabDocs/master/protocols/Commercial_Protocols/ThermoFisher_OgeneRuler_DNA_Ladder_Mix_F100439.jpg)(https://raw.githubusercontent.com/sr320/LabDocs/master/protocols/Commercial_Protocols/ThermoFisher_OgeneRuler_DNA_Ladder_Mix_F100439.jpg)[![](http://eagle.fish.washington.edu/Arabidopsis/20150915_gel_gDNA_oly_geoduck.jpg)](http://eagle.fish.washington.edu/Arabidopsis/20150915_gel_gDNA_oly_geoduck.jpg)











The gel is loaded in the order listed above (going left to right on the gel).

All samples look really good with prominent high molecular weight bands and little smearing.