--- author: Sam White toc-title: Contents toc-depth: 5 toc-location: left date: 2015-09-16 20:17:57+00:00 layout: post slug: agarose-gel-geoduck-gdna-integrity-check title: Agarose Gel – Geoduck gDNA Integrity Check categories: - 2015 - Geoduck Genome Sequencing tags: - gDNA - gel - geoduck - O'geneRuler DNA Ladder Mix - Panopea generosa --- Ran 0.8% agarose 1x modified TAE gel stained with EtBr to assess the integrity of [geoduck gDNA isolated earlier today](https://robertslab.github.io/sams-notebook/posts/2015/2015-09-16-genomic-dna-isolation-geoduck-adductor-muscle-foot-4/). Ran ~500ng of each sample: Geoduck adductor muscle 1: 4μL Results: ![](https://raw.githubusercontent.com/sr320/LabDocs/master/protocols/Commercial_Protocols/ThermoFisher_OgeneRuler_DNA_Ladder_Mix_F100439.jpg)(https://raw.githubusercontent.com/sr320/LabDocs/master/protocols/Commercial_Protocols/ThermoFisher_OgeneRuler_DNA_Ladder_Mix_F100439.jpg)[![](http://eagle.fish.washington.edu/Arabidopsis/20150916_gel_gDNA_geoduck.jpg)](http://eagle.fish.washington.edu/Arabidopsis/20150916_gel_gDNA_geoduck.jpg) The gDNA looks really good with a prominent high molecular weight band and little smearing. Will proceed with pooling all accumulated geoduck gDNA for this project.