---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2015-08-19 23:11:21+00:00
layout: post
slug: rad-seq-library-prep-reagents
title: RAD-Seq Library Prep Reagents
categories:
  - 2015
  - 2bRAD Library Tests for Sequencing at Genewiz
  - Reagent Prep
tags:
  - AlfI
  - RAD-seq
---

A box with the above title was established in the -20C in FTR 209 containing the following:




    
  * Thermo Scientific AlfI: ER1801

    
  * NEB T4 DNA ligase, 50 μL: M0202S

    
  * NEB 10 mM ATP, 1 mL: P0756S

    
  * Promega dNTPs (10 mM each): U1511

    
  * NEB Q5 Taq Polymerase, 100 units: M0491S



Oligos (100μL each in TE pH=8.0; barcode sequences are in bold)

Adaptor 1
5ILL-NR: CTACACGACGCTCTTCCGATCTNR

Anti-ILL: AGATCGGAAGAGC(InvdT)

Adaptor 2
3ILL-NR: CAGACGTGTGCTCTTCCGATCTNR

ILL-Lib1: AATGATACGGCGACCACCGA

ILL-Lib2: CAAGCAGAAGACGGCATACGA

ILL-HT1: AATGATACGGCGACCACCGAGATCTACAC**ATGCAT**ACACTCTTTCCCTACACGACGCTCTTCCGATCT

ILL-HT2:AATGATACGGCGACCACCGAGATCTACAC**CGTACG**ACACTCTTTCCCTACACGACGCTCTTCCGATCT

ILL-BC1: CAAGCAGAAGACGGCATACGAGAT**CGTGAT**GTGACTGGAGTTCAGACGTGTGCTCTTCCGATC