---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2015-08-06 13:41:31+00:00
layout: post
slug: rna-quantification-o-lurida-1hr-post-mechanical-heat-stress-dnased-rna
title: RNA Quantification - O.lurida 1hr post-mechanical heat stress DNased RNA
categories:
  - 2015
  - Olympia oyster reciprocal transplant
tags:
  - ctenidia
  - DNased RNA
  - gill
  - mechanical stress
  - NanoDrop1000
  - olympia oyster
  - Ostrea lurida
  - RNA quantification
---

[DNased RNA from 07272015](https://robertslab.github.io/sams-notebook/posts/2015/2015-07-27-dnase-treatment-o-lurida-ctenidia-1hr-post-mechanical-stress-rna/) was quantified using the Roberts Lab NanoDrop1000.



Results:

The 260/280 ratios don't look great, but that is most likely due to the DNase treatment. The DNase that's added to each sample isn't actually removed, so that additional protein will skew the 260/280 ratios. Will proceed with qPCR to check for any residual gDNA in these samples.



![](https://eagle.fish.washington.edu/Arabidopsis/20150806_Jake_oly_mech_stress_DNasedRNA_ODs.JPG)(http://eagle.fish.washington.edu/Arabidopsis/20150806_Jake_oly_mech_stress_DNasedRNA_ODs.JPG)

![](https://eagle.fish.washington.edu/Arabidopsis/20150806_Jake_oly_mech_stress_DNasedRNA_plots_01.JPG)(http://eagle.fish.washington.edu/Arabidopsis/20150806_Jake_oly_mech_stress_DNasedRNA_plots_01.JPG)

![](https://eagle.fish.washington.edu/Arabidopsis/20150806_Jake_oly_mech_stress_DNasedRNA_plots_02.JPG)(http://eagle.fish.washington.edu/Arabidopsis/20150806_Jake_oly_mech_stress_DNasedRNA_plots_02.JPG)
