--- author: Sam White toc-title: Contents toc-depth: 5 toc-location: left date: 2015-08-03 15:47:43+00:00 layout: post slug: goals-august-2015 title: Goals - August 2015 categories: - 2015 - Goals --- ### Review of [last month's goals](https://robertslab.github.io/sams-notebook/posts/2015/2015-07-02-goals-july-2015/): #### _O.lurida_ RNA Isolation & Reverse Transcription - RNA has been isolated and DNased from Jake's mechanically stressed samples. #### Miscellany - Improved organization of -80C, added frequently used protocols to [the Roberts Lab GitHub Wiki](https://github.com/sr320/LabDocs/wiki), connected BGI rep with purchasing to get the PO situation figured out for Olympia oyster and geoduck genome sequencing, sent samples to BGI for Olympia oyster genome-by-sequencing (GBS). * * * ###  _O.lurida_ Reverse Transcription Need to quantify the DNased RNA from Jake's mechanically stressed oysters and then verify that the DNase treatment worked. Will then proceed with reverse transcription. ### Miscellany Continue work on -80C organization, continue creating "readme" files for folders on our server(s), continue migration from Wikispaces to GitHub, attempt to combine our PrimerDatabase and our Primer Stocks spreadsheets into a single document (and create a SQL database from that), fix the shortfalls from our EH&S lab inspection.