---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2015-07-16 00:46:42+00:00
layout: post
slug: rna-isolation-o-lurida-ctenidia-1hr-post-mechanical-stress-2
title: RNA Isolation – O.lurida Ctenidia 1hr Post-Mechanical Stress
categories:
  - 2015
  - Olympia oyster reciprocal transplant
tags:
  - ctenidia
  - gill
  - mechanical stress
  - olympia oyster
  - Ostrea lurida
  - RNA isolation
  - RNAzol RT
---

Isolated RNA from [Jake’s _Ostrea lurida_ ctenidia samples that had been subjected to mechanical stress (from 20150422)(https://heareresearch.blogspot.com/2015/04/4-22-2015-heatmechanical-shock.html).

Despite the indication in this notebook, the samples had not been previously homogenized in RNAzol RT. I thawed the samples, homogenized them and followed the [RNAzol RT protocol for total RNA isolation](https://github.com/sr320/LabDocs/blob/master/protocols/Commercial_Protocols/MRC_RNAzol-RT-May-2014.pdf). Here’s the list of samples:




    
  * 42215 HM1 1

    
  * 42215 HM1 2

    
  * 42215 HM1 3

    
  * 42215 HM1 4

    
  * 42215 HM1 5

    
  * 42215 HM1 6

    
  * 42215 HM1 7

    
  * 42215 HM1 8

    
  * 42215 SM1 1

    
  * 42215 SM1 2

    
  * 42215 SM1 3

    
  * 42215 SM1 4

    
  * 42215 SM1 5

    
  * 42215 SM1 6

    
  * 42215 SM1 7

    
  * 42215 SM1 8



RNA was resuspended in 50μL of 0.1%DEPC-H2O and stored @ -80C in the original box they came from.