---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2015-04-24 16:09:57+00:00
layout: post
slug: bioanalyzer-data-geoduck-rna-from-histology-blocks
title: Bioanalyzer Data - Geoduck RNA from Histology Blocks
categories:
  - 2015
  - Protein expression profiles during sexual maturation in Geoduck
tags:
  - bioanalyzer
  - electropherogram
  - foot
  - gel
  - geoduck
  - Panopea generosa
  - RNA
  - RNA Pico
---

I received the Bioanalyzer data back for the [geoduck foot RNA samples I submitted 20150422](https://robertslab.github.io/sams-notebook/posts/2015/2015-04-22-bioanalyzer-submission-geoduck-rna-from-histology-blocks/). The two samples were run on the RNA Pico chip assay.



Results:

Bioanalzyer 2100 Data File (XAD): [SamWhite_Eukaryote Total RNA Pico_2015-04-23_13-04-16.xad](https://eagle.fish.washington.edu/Arabidopsis/Bioanalyzer%20Data/SamWhite_Eukaryote%20Total%20RNA%20Pico_2015-04-23_13-04-16.xad)

Data file requires 2100_Expert_B0208_SI648_SR2 version of the software (Windows).



### Gel Representation



![](https://eagle.fish.washington.edu/Arabidopsis/Bioanalyzer%20Data/20150423_pico_bioanlayzer_geoduck_gel.jpg)(http://eagle.fish.washington.edu/Arabidopsis/Bioanalyzer%20Data/20150423_pico_bioanlayzer_geoduck_gel.jpg)





### Electropherogram



![](https://eagle.fish.washington.edu/Arabidopsis/Bioanalyzer%20Data/20150423_pico_bioanlayzer_geoduck_electropherogram.jpg)(http://eagle.fish.washington.edu/Arabidopsis/Bioanalyzer%20Data/20150423_pico_bioanlayzer_geoduck_electropherogram.jpg)



The samples look really good! As we've seen previously in shellfish RNA, there is a single, prominent rRNA band/peak with very little degradation (smearing and/or no prominent peak/band).

Will proceed with RNA isolation from the remaining histology blocks.
