---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2015-01-28 04:03:20+00:00
layout: post
slug: bisuflite-ngs-library-prep-c-gigas-larvae-oa-bisulfite-dna-continued-from-yesterday
title: Bisuflite NGS Library Prep - C.gigas larvae OA bisulfite DNA (continued from
  yesterday)
categories:
  - 2015
  - Crassostrea gigas larvae OA (2011) bisulfite sequencing
tags:
  - barcodes
  - BS-seq
  - Crassostrea gigas
  - library prep
  - Pacific oyster
---

Continued Illumina library prep of bisulfite-treated DNA samples (400ppm and 1000ppm; from [20150114)(2015/01/14/dna-bisulfite-conversion-c-gigas-larvae-oa-sheared-dna.html)  with [Methylamp DNA Modification Kit (Epigentek)](https://github.com/sr320/LabDocs/blob/master/protocols/Commercial_Protocols/Epigentek_PostBisulfiteIlluminaLibraryPrep_P-1055.pdf). Performed bead clean up immediately after End Repair.

PCR cycles: 14

No other changes were made to the manufacturer's protocol.

Epigentek Barcode Indices assigned, per their recommendations for using two libraries for multiplexing:

400ppm - barcode #6 - GCCAAT

1000ppm - barcode #12 - CTTGTA

The two libraries were stored @ -20C and will be quantified tomorrow.


