---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2015-01-27 00:52:57+00:00
layout: post
slug: bisuflite-ngs-library-c-gigas-larvae-oa-bisulfite-dna
title: Bisuflite NGS Library Prep - C.gigas larvae OA bisulfite DNA
categories:
  - 2015
  - Crassostrea gigas larvae OA (2011) bisulfite sequencing
tags:
  - Crassostrea gigas
  - larvae
  - library prep
  - Methylamp DNA Modification
  - OA
  - ocean acidification
  - Pacific oyster
---

The two pooled bisulfite-treated DNA samples (400ppm and 1000ppm) from [20150114](https://robertslab.github.io/sams-notebook/posts/2015/2015-01-15-dna-bisulfite-conversion-c-gigas-larvae-oa-sheared-dna/) were used to prepare bisulfite Illumina libraries with [EpiNext Post-Bisulfite DNA Library Preparation Kit (Illumina) (Epigentek)(https://github.com/sr320/LabDocs/blob/master/protocols/Commercial_Protocols/Epigentek_PostBisulfiteIlluminaLibraryPrep_P-1055.pdf).  Changes to the manufacturer's protocol:




    
  * Samples were transferred to 1.5mL snap cap tubes for all magnetic bead steps in order to fit in our tube magnets.

    
  * Stopped after End Repair step (prior to magnetic bead clean up).  Samples stored @ -20C


