---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2015-01-15 04:45:10+00:00
layout: post
slug: dna-bisulfite-conversion-c-gigas-larvae-oa-sheared-dna
title: DNA Bisulfite Conversion - C.gigas larvae OA Sheared DNA
categories:
  - 2015
  - Crassostrea gigas larvae OA (2011) bisulfite sequencing
tags:
  - bisulfite conversion
  - DNA
---

After discussing with Steven, decided to use only samples from 20110513, due to high algae amounts present in the 20110519 samples.

Pooled the DNA of the 400ppm samples (6B2 & 6B5) and pooled the DNA of the 1000ppm samples(1B1 & 1B2) , for a total of two samples. 50ng of each sample was used to make the pools, for a toal of 100ng of DNA in each pool. Calculations are below:
[https://docs.google.com/spreadsheets/d/1e7EF05akWeBtO7Xz0UWXhIzRlkVU5HA_rjCE3c4SPEw/edit?usp=sharing](https://docs.google.com/spreadsheets/d/1e7EF05akWeBtO7Xz0UWXhIzRlkVU5HA_rjCE3c4SPEw/edit?usp=sharing)

``

Each pooled sample was brought up to a final volume of 24μL for use in bisulfite conversion kit.

Performed bisulfite conversion of sheared DNA from [20150113](https://robertslab.github.io/sams-notebook/posts/2015/2015-01-13-speedvac-c-gigas-larvae-oa-dna/) using the Methylamp DNA Modification Kit (Epigentek) according the manufacturer's protocol.

Samples were eluted with 10μL of Buffer R6 and stored @ -20C.



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