---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2015-01-13 00:12:01+00:00
layout: post
slug: speedvac-c-gigas-larvae-oa-dna
title: SpeedVac - C.gigas larvae OA DNA
categories:
  - 2015
  - Crassostrea gigas larvae OA (2011) bisulfite sequencing
tags:
  - concentrate
  - Crassostrea gigas
  - DNA
  - SpeedVac
---

The DNA extracted and sheared on [20150109](https://robertslab.github.io/sams-notebook/posts/2015/2015-01-09-dna-isolation-c-gigas-larvae-from-2011-noaa-oa-experiment/) was in a volume too great to proceed with bisulfite conversion.  Dried the samples to complete dryness in a SpeedVac (~4hrs).  Reconsitituted DNA in 24μL of NanoPure water.  Will bisulfite convert tomorrow.

Sample list:
<table width="263" style="height: 244px;" >
<tbody >
<tr >

<td style="text-align: center;" >SAMPLE ID
</td>

<td style="text-align: center;" >DATE
</td>

<td style="text-align: center;" >TREATMENT (ppm)
</td>

<td style="text-align: center;" ># LARVAE
</td>

<td style="text-align: center;" >TOTAL DNA (ng)
</td>
</tr>
<tr >

<td style="text-align: center;" >6B5
</td>

<td style="text-align: center;" >20110513
</td>

<td style="text-align: center;" >400
</td>

<td style="text-align: center;" >5,000
</td>

<td style="text-align: center;" >105
</td>
</tr>
<tr >

<td style="text-align: center;" >1B2
</td>

<td style="text-align: center;" >20110513
</td>

<td style="text-align: center;" >1000
</td>

<td style="text-align: center;" >5,000
</td>

<td style="text-align: center;" >109
</td>
</tr>
<tr >

<td style="text-align: center;" >6B2
</td>

<td style="text-align: center;" >20110513
</td>

<td style="text-align: center;" >400
</td>

<td style="text-align: center;" >10,000
</td>

<td style="text-align: center;" >117
</td>
</tr>
<tr >

<td style="text-align: center;" >1B1
</td>

<td style="text-align: center;" >20110513
</td>

<td style="text-align: center;" >1000
</td>

<td style="text-align: center;" >10,000
</td>

<td style="text-align: center;" >593
</td>
</tr>
<tr >

<td style="text-align: center;" >1B1
</td>

<td style="text-align: center;" >20110519
</td>

<td style="text-align: center;" >1000
</td>

<td style="text-align: center;" >NA
</td>

<td style="text-align: center;" >500
</td>
</tr>
<tr >

<td style="text-align: center;" >1B2
</td>

<td style="text-align: center;" >20110519
</td>

<td style="text-align: center;" >1000
</td>

<td style="text-align: center;" >NA
</td>

<td style="text-align: center;" >500
</td>
</tr>
<tr >

<td style="text-align: center;" >6B2
</td>

<td style="text-align: center;" >20110519
</td>

<td style="text-align: center;" >400
</td>

<td style="text-align: center;" >NA
</td>

<td style="text-align: center;" >269
</td>
</tr>
<tr >

<td style="text-align: center;" >6B1
</td>

<td style="text-align: center;" >20110519
</td>

<td style="text-align: center;" >400
</td>

<td style="text-align: center;" >NA
</td>

<td style="text-align: center;" >345
</td>
</tr>
</tbody>
</table>


Updated the quantification spreadsheet with a column labeled "[New](ug/uL)" that calculates the new concentrations of the above samples in the 24μL volume.

[https://docs.google.com/spreadsheets/d/1e7EF05akWeBtO7Xz0UWXhIzRlkVU5HA_rjCE3c4SPEw/edit?usp=sharing](https://docs.google.com/spreadsheets/d/1e7EF05akWeBtO7Xz0UWXhIzRlkVU5HA_rjCE3c4SPEw/edit?usp=sharing)

``



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