---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2014-09-23 04:49:13+00:00
layout: post
slug: dna-isolation-c-gigas-larvae-from-katie-latterhos-and-emma
title: DNA Isolation - C.gigas Larvae from Katie Latterhos and Emma
categories:
  - 2014
  - Miscellaneous
tags:
  - Crassostrea gigas
  - DNA Isolation
  - DNA Quantification
  - larvae
  - OA
  - ocean acidification
---

Isolated gDNA from two C.gigas larvae samples (stored in RNA Later) from Katie Latterhos:

B4 400 D05

B6 400 D03

and two samples from Emma:

280E

380E

Emma's samples were in her -80C box (in rack #2): C.gigas larvae - NOAA O.A. September 2010 Emma

Note: No visible larvae present in either of Katie Latterhos samples. Easily visible larvae in each of Emma's samples.

DNA was isolated using the Quick-gDNA MicroPrep Kit (ZymoResearch) according to the manufacturer's protocol for Cell Suspensions and Proteinase K Digested Samples.

Samples were eluted with 10uL of elution buffer and spec'd on a NanoDrop1000 (ThermoFisher). The 2uL used for each sample were recovered from the NanoDrop.

Results:

![](https://eagle.fish.washington.edu/Arabidopsis/20140922%20-%20Gigas%20gDNA%20ODs-01.JPG)

Not surprisingly, the samples from Katie Latterhos yielded, essentially, no gDNA. Will discuss with Steven.

*UPDATE 20141030*

Steven sent me this screen cap of Emma's notebook so we could track where the samples originated from

![](https://www.evernote.com/shard/s10/sh/5e19089d-0c31-426b-844a-e46249fac4e4/907d17586bd2b4eb0603686c9560f0dd/deep/0/genefish---Emma%27s-Notebook.png)