---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2013-03-14 22:20:18+00:00
layout: post
slug: pcr-hexokinase-promoter-and-cds-repeat-from-20130227
title: PCR - Hexokinase Promoter and CDS (repeat from 20130227)
categories:
  - 2013
  - Miscellaneous
tags:
  - Crassostrea gigas
  - hexokinase
  - Pacific oyster
  - PCR
---

Performed a repeat of the failed PCR from 20130227, but used a pool of cDNA (made from 20110311 C.gigas cDNA) instead of a single sample and changed the annealing temp to 50C.

Results:

Same exact results as 20130227; nothing. As such, didn't take gel image. Will retry one more time using a long-distance polymerase, along with varying [MgCl2].

UPDATE 20130318: Doh! When talking about this at lab meeting today, I realized I'm trying to amplify the promoter (a genomic sequence) using cDNA! Will re-design primers and develop new cloning strategy for this!