--- author: Sam White toc-title: Contents toc-depth: 5 toc-location: left date: 2012-07-23 23:23:35+00:00 layout: post slug: minipreps-emmas-illumina-library-cloning title: Minipreps - Emma's Illumina Library Cloning categories: - 2012 - Miscellaneous tags: - DNA - plasmid isolation - QIAprep --- Performed plasmid isolation on 17 bacterial cultures Emma inoculated yesterday using the QIAprep Spin Mini Kit (Qiagen) using ~1.4mL of culture according to the manufacturer's protocol. Plasmid DNA was eluted with 50uL of Buffer EB. Tubes were stored @ 4C in the refrigerator in FTR 213.