---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2012-07-11 23:28:04+00:00
layout: post
slug: illumina-rnaseq-library-construction-32-c-gigas-individuals
title: Illumina RNAseq Library Construction - 32 C.gigas Individuals
categories:
  - 2012
  - Miscellaneous
tags:
  - Crassostrea gigas
  - Illumina
  - library prep
  - Pacific oyster
  - RNA-seq
---

Took heat-fragmented RNA provided by Emma (see Emma's Notebook, 7/3/2011) and proceeded to make first strand cDNA, as described in the [Eli Meyer protocol for Illumina HiSeq](https://genefish.fish.washington.edu/~srlab/Steven/Lab%20Protocols/RNASeq%20sample%20prep%20-%20HiSeq%20-%2004%20Apr%202012.pdf). [Master mix calcs are here](http://eagle.fish.washington.edu/Arabidopsis//Notebook%20Workup%20Files/20120711-01.jpg). Samples were stored @ -20C after the reverse transcription and library construction will be continued tomorrow.