---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2012-04-28 00:05:28+00:00
layout: post
slug: qpcr-check-dnased-rna-from-earlier-today-for-residual-gdna
title: qPCR - Check DNased RNA from Earlier Today for Residual gDNA
categories:
  - 2012
  - Miscellaneous
tags:
  - CFX96
  - Crassostrea gigas
  - DNased RNA
  - larvae
  - Pacific oyster
  - qPCR
  - Vibrio tubiashii
  - VtpA
---

Ran qPCR using V.tubiashii VtpA primers (from Elene; no SR ID). Used 0.5uL of each DNased RNA sample, which equals ~40ng of RNA, which would be the equivalent amount of RNA that would end up in a qPCR rxn after cDNA has been made (using 1uL of cDNA). Used the filter DNA extraction from samples #279 from DATE as a positive control. Master mix calcs are here. Plate layout, cycling params, etc. can be found in the qPCR Report (see Results).

Results:

[qPCR Data File](https://eagle.fish.washington.edu/Arabidopsis/qpcr/CFX96/Roberts%20Lab_2012-04-27%2013-31-42_CC009827.pcrd) (CFX96)

[qPCR Report](https://eagle.fish.washington.edu/Arabidopsis/qpcr/CFX96/Roberts%20Lab_2012-04-27%2013-31-42_CC009827.pdf) (PDF)

All samples showed up negative, except for the positive control. Will proceed with making cDNA on Monday.