---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2012-04-27 00:07:39+00:00
layout: post
slug: rna-isolation-c-gigas-larvae-from-taylor-summer-2011
title: RNA Isolation - C.gigas Larvae from Taylor Summer 2011
categories:
  - 2012
  - Miscellaneous
tags:
  - Crassostrea gigas
  - larvae
  - NanoDrop1000
  - Pacific oyster
  - RNA
  - RNA isolation
  - RNA quantification
  - RNAlater
  - TriReagent
---

Samples had been stored in RNA Later (Ambion). Samples were pelleted and the RNA Later supe removed. Samples were washed (2x) with 1mL TE (pH = 8.0) to remove excess salt resulting from the RNA Later. Samples were split, roughly equally, into two separate tubes. Samples were pelleted and the supe removed. One tube from each sample was set aside for gDNA isolation using DNAzol (MRC). The other tube was vortexed vigorously in TriReagent (MRC) and the then treated according to protocol. Samples were resuspended in 100uL of 0.1% DEPC-H2O and spec'd on the Roberts Lab NanoDrop 1000.

Results:

![](https://eagle.fish.washington.edu/Arabidopsis/RNA%20Spec%20Readings/20120426%20RNA%20ODs-01.JPG)

Overall, the samples look really good. Some samples (280, 434 & 605) required re-specing after the NanoDrop was reblanked in order to get a reading without an error message. They will be DNased and then reverse transcribed.