---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2012-04-27 00:11:40+00:00
layout: post
slug: gdna-isolation-c-gigas-larvae-from-taylor-summer-2011
title: gDNA Isolation - C.gigas Larvae from Taylor Summer 2011
categories:
  - 2012
  - Miscellaneous
tags:
  - Crassostrea gigas
  - DNA Isolation
  - DNA Quantification
  - DNazol
  - gDNA
  - larvae
  - NanoDrop1000
  - Pacific oyster
---

Samples that had been split from earlier today (see the RNA Isolation below) were resuspended in 1mL of DNAzol (MRC). 100ug of Proteinase K (Fermentas) was added to each sample. Samples were incubated at RT, O/N on a rotator. On 20120427 samples were pelleted 10mins, 10,000g, and supe transferred to fresh tube. DNA was precipitated with 0.5mL of 100% EtOH, mixed gently and pelleted 5mins, 5000g. Supe was discarded, pellets were washed with 1mL 75% EtOH, re-pelleted at same speed as previous step, supe discarded and pellets were resuspended in NanoPure H2O. Samples were spec'd on the Roberts Lab NanoDrop1000.

Results:

Report on the NanoDrop software wouldn't display, so I've entered the concentration of each sample in the table below.

<table class="wiki_table mceItemTable" >
<tbody >
<tr >

<td >SampleID
</td>

<td >ng/uL
</td>
</tr>
<tr >

<td >201
</td>

<td >1364
</td>
</tr>
<tr >

<td >280
</td>

<td >131.7
</td>
</tr>
<tr >

<td >314
</td>

<td >710.3
</td>
</tr>
<tr >

<td >342
</td>

<td >539.4
</td>
</tr>
<tr >

<td >434
</td>

<td >274.4
</td>
</tr>
<tr >

<td >552
</td>

<td >334.8
</td>
</tr>
<tr >

<td >605
</td>

<td >369.7
</td>
</tr>
</tbody>
</table>