---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2012-03-24 00:18:22+00:00
layout: post
slug: qpcr-taylor-water-filter-dna-extracts-from-yesterday
title: qPCR - Taylor Water Filter DNA Extracts from Yesterday
categories:
  - 2012
  - Miscellaneous
tags:
  - 16s
  - CFX96
  - qPCR
  - RE22
  - Vibrio tubiashii
  - water filter
---

Ran qPCR on the Taylor water filter DNA extracts from yesterday using V.tubiashii 16s primers (SR IDs: 455, 456). Used RE22 DNA as a positive control, provided by Elene. [Master mix calcs are here](https://eagle.fish.washington.edu/Arabidopsis//Notebook%20Workup%20Files/20120323-01.jpg). All samples were run in duplicate. Plate layout, cycling params, etc can be found in the qPCR Report (see Results).

Results:

[qPCR Data File](https://eagle.fish.washington.edu/Arabidopsis/qPCR/CFX96/Roberts%20Lab_2012-03-23%2009-25-17_CC009827.pcrd) (CFX96)

[qPCR Report](https://eagle.fish.washington.edu/Arabidopsis/qPCR/CFX96/Roberts%20Lab_2012-03-23%2009-25-17_CC009827.pdf) (PDF)

All samples amplified, including the negative controls. Negative controls exhibited very weak, late amplification. Additionally, many of the samples have a "shoulder" or apparent double-peak present in the melt curves. Will repeat to see if I can eliminate amplification in negative control samples.
