---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2012-03-13 00:25:22+00:00
layout: post
slug: reverse-transcription-daves-manila-clam-venerupis-philippinarum-dnased-rna-from-20120307-and-20120302
title: Reverse Transcription - Dave's Manila Clam (Venerupis philippinarum) DNased
  RNA from 20120307 and 20120302
categories:
  - 2012
  - Miscellaneous
tags:
  - DNased RNA
  - M-MLV
  - Manila clam
  - reverse transcription
  - Venerupis philippinarum
---

Performed reverse transcription on 1.5ug of DNased RNA in a 75uL reaction, using oligo dT primers. All reagents were scaled appropriately (based on Promega's M-MLV RT protocol). Samples were prepared in a plate and stored @ -20C. Plate layout and all reverse transcription calcs are here:
