---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2012-03-08 01:33:59+00:00
layout: post
slug: rna-isolation-daves-manila-clam-venerupis-philippinarum-gill-samples-25-48
title: RNA Isolation - Dave's Manila Clam (Venerupis philippinarum) Gill Samples (#25-48)
categories:
  - 2012
  - Miscellaneous
tags:
  - DNase
  - DNased RNA
  - Manila clam
  - NanoDrop1000
  - RNA isolation
  - RNA quantification
  - TriReagent
  - Turbo DNA-free
  - Venerupis philippinarum
---

Isolated RNA from Manila Clam gill samples provided by Dave, according to protocol. Samples were resuspended in 0.1%-DEPC H2O and spec'd on the Roberts Lab NanoDrop1000. Samples were stored @ -80C in Dave's box that the tissue was initially stored in.

Results:

![](https://eagle.fish.washington.edu/Arabidopsis//RNA%20Spec%20Readings/20120307%20-%20RNA%20Vp%20gill%20ODs-01.JPG)

All samples look great with excellent yields and great 260/280 values. Will proceed with DNasing. (Note: Sample #42 appears twice because the first reading had an air bubble and, as such, should be discarded.)



DNased RNA using Ambion's Turbo DNA-free Kit following the "routine" protocol. 5ug of total RNA from each sample was treated in 50uL reactions. Samples will be spec'd on Monday with the Roberts Lab NanoDrop 1000.

Results:

![](https://eagle.fish.washington.edu/Arabidopsis//RNA%20Spec%20Readings/20120307%20-%20DNased%20RNA%20Vp%20gill%20ODs-01.JPG)