---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2012-01-26 01:54:15+00:00
layout: post
slug: dnase-c-gigas-rna-from-20120124
title: DNAse - C.gigas RNA from 20120124
categories:
  - 2012
  - Miscellaneous
tags:
  - Crassostrea gigas
  - DNase
  - NanoDrop1000
  - Pacific oyster
  - RNA
  - RNA quantification
  - Turbo DNA-free
---

5ug of each RNA was DNased using Ambion's Turbo DNA-free Kit, according to the rigorous protocol and spec'd on Roberts Lab NanoDrop 1000. [RNA volume calcs are here](https://docs.google.com/spreadsheet/ccc?key=0AmS_90rPaQMzdEJBaExkeTVQb1BoWFkyaHNLQnN0T0E#gid=0).

Results:

![](https://eagle.fish.washington.edu/Arabidopsis//RNA%20Spec%20Readings/20120125%20-%20DNased%20RNA%20ODs-01.JPG)

DNased RNA looks fine. Low OD260/280 ratios, but this is often seen after DNase treatment and particularly with low [RNA]. Will perform qPCR to assess gDNA removal.
