---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2012-01-25 01:59:18+00:00
layout: post
slug: rna-isolation-c-gigas-larvae-from-20110412-20110705-continued-from-20120112
title: RNA Isolation - C.gigas Larvae from 20110412 & 20110705 (Continued from 20120112)
categories:
  - 2012
  - Miscellaneous
tags:
  - EtOH precipitation
  - NanoDrop1000
  - RNA
  - RNA isolation
  - RNA quantification
  - TriReagent
---

All of the RNA samples were re-combined with their respective counterparts and subject to a standard EtOH precipitation (0.1 volumes of 3M NaOAc, pH = 5.2, 2.5 volumes 100% EtOH; incubated -80C 1hr; pelleted; washed with 1mL 70% EtOH; pelleted). Pellets were washed two additional times (for a total of three washes) with 70% EtOH. RNA was resuspended in 50uL of 0.1% DEPC-H2O and spec'd on the Roberts Lab NanoDrop 1000.

Results:

![](https://eagle.fish.washington.edu/Arabidopsis//RNA%20Spec%20Readings/20120124%20Gigas%20Larvae%20RNA%20ODs-01.JPG)

Yields for the 4/12/2011 samples were all lower than the yields for the 7/5/2011 samples. However, the RNA quality (based on OD260/280 ratios) looks pretty good for both groups of RNA. RNA will be treated with DNase before reverse transcription.