---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2011-10-07 02:26:06+00:00
layout: post
slug: pcr-purified-coxpgs-12-dna-from-earlier-today
title: PCR - Purified COX/PGS 1/2 DNA from earlier today
categories:
  - 2011
  - Miscellaneous
tags:
  - COX
  - COX1
  - COX2
  - Crassostrea gigas
  - cyclooxygenase
  - gel
  - Pacific oyster
  - PCR
  - PGS
  - PGS1
  - PGS2
  - prostaglandin synthase
---

Ran PCR using primers Cg_COX1/2_qPCR_F, Cg_COX1_qPCR_R, Cg_COX2_454align1_R (SR IDs: 1192, 1191, 1190; respectively). Template was pooled cDNA from 20110311 of various C.gigas tissues. These reactions will verify (sort of) if we have both isoforms present in the PCR performed earlier today, prior to cloning. [Master mix calcs and cycling params are here](https://eagle.fish.washington.edu/Arabidopsis/20111006-03.jpg).

Results:

![](https://eagle.fish.washington.edu/Arabidopsis/20111007-01.JPG)(http://eagle.fish.washington.edu/Arabidopsis/20111007-01.JPG)

Lane 1: Hyperladder I (Bioline)

Lane 2: COX1/PGS1 primer set

Lane 3: COX1/PGS1 primer set NTC

Lane 4: COX2/PGS2 primer set

Lane 5: COX2/PGS2 primer set NTC

NTCs are clean for both primer sets. We see bands of the expected size for both primer sets. Additionally, we see lower expression in COX2/PGS2, as we observed in our previous qPCR reactions with these primer sets. Will clone the large fragment that was PCR'ed/purified from earlier today.