---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2011-09-21 02:39:44+00:00
layout: post
slug: pcr-full-length-pgs1-cdna-2
title: PCR - Full-length PGS1 cDNA
categories:
  - 2011
  - Miscellaneous
tags:
  - cDNA
  - Crassostrea gigas
  - gel
  - Pacific oyster
  - PCR
  - PGS
  - PGS1
  - prostaglandin synthase
---

Need more PCR product for sequencing. Repeated reaction from 20110825.

Results:

![](https://eagle.fish.washington.edu/Arabidopsis/20110921-01.JPG)

Lane 1 - Hypperladder I (Bioline)

Lane 2 - PCR 1 & 2

Lane 3 - PCR 3 & 4

Lane 4 - PCR 5 & 6

Lane 5 - Neg. Control

Bands from lanes 2 - 4 were excised and purified with Ultra-free DA columns (Millipore) and spec'd. Concentration was extremely low (3.5ng/uL) and too dilute for sequencing. Will EtOH precipitate.
