---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2011-08-05 02:56:57+00:00
layout: post
slug: sequencing-c-gigas-cox2pgs2-clone-4-from-20110728
title: 'Sequencing - C.gigas COX2/PGS2 Clone #4 from 20110728'
categories:
  - 2011
  - Miscellaneous
tags:
  - COX2
  - Crassostrea gigas
  - cyclooxygenase
  - Pacific oyster
  - PGS2
  - prostaglandin synthase
  - Sanger sequencing
---

Used new primers for sequencing (SR IDs: 1351 & 1352) clone #4. Sequenced clone two times in each direction. DNA and primers were sent for sequencing at ASU. Requested "High GC" treatment to help overcome the issue seen on 20110728.

Results:

Sequencing results received 20110810. Initial analysis suggests that we managed to fully sequence this clone! Will try to assemble a full-length CDS for COX2/PGS2.
