---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2011-07-26 03:12:21+00:00
layout: post
slug: cloning-c-gigas-cox2pgs2-53-race-products-from-earlier-today
title: Cloning - C.gigas COX2/PGS2 5'/3' RACE Products (from earlier today)
categories:
  - 2011
  - Miscellaneous
tags:
  - COX
  - COX2
  - Crassostrea gigas
  - cyclooxygenase
  - kanamycin
  - ligation
  - Pacific oyster
  - pCR2.1
  - PGS
  - PGS2
  - prostaglandin synthase
  - TOPO TA Cloning
---

The bands that were excised and purified earlier today were cloned in to pCR2.1 using the TOPO TA Cloning Kit (Invitrogen) according to the manufacturer's protocol with the following changes: used 4uL of all PCR products, incubated ligation reactions for 15mins @ RT, incubated competent cells with ligation reactions for 15mins on ice.

Two volumes of each reaction were plated (50uL and 100uL) on Kan50 plates with X-gal (made 20010412 by SJW) and incubated @ 37C O/N.

Results:

Ample number of white colonies for all 4 sets of cloning reactions.