---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2011-07-16 03:19:06+00:00
layout: post
slug: sequencing-pgs-hi-4-pgs2cox2
title: Sequencing - PGS Hi 4 (PGS2/COX2)
categories:
  - 2011
  - Miscellaneous
tags:
  - alignment
  - BLAST
  - COX
  - COX2
  - Crassostrea gigas
  - cyclooxygenase
  - Pacific oyster
  - PGS
  - PGS2
  - prostaglandin synthase
  - Sanger sequencing
---

Sent plasmid prep to ASU (5uL of plasmid + 1uL of 10uM M13F/R). SJW01 = M13F, SJW02 = M13R.

Results:

Sequencing looks great! Definitely have a portion of the second isoform of COX/PGS!! Here's the result of the consensus BLASTed in GenBank>Nucleotide (others)>blastn:

![](https://eagle.fish.washington.edu/Arabidopsis/20110721%20PGS2%20BLAST%20Alignment.png)

Top hit in the db is COX1/PGS1, and, clearly, there are differences between the two sequences confirming that we have the second isoform (COX2/PGS2). Will design more RACE primers in hopes of obtaining the full-length cDNA sequence.